Journal: PLoS ONE
Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
doi: 10.1371/journal.pone.0221123
Figure Lengend Snippet: A. Control of samples found positive for BRAF mutation by ASA/Sequencing but negative by IHC. B. Control of samples found negative for BRAF mutation by ASA/sequencing and positive by IHC (wrong chromogen used). C. Control of samples found positive for NRAS mutation by sequencing but negative by Idylla. Yellow dots correspond to wild type DNA copies ( BRAF , panels A and B; NRAS , panel C). Green dots correspond to mutated DNA copies ( BRAF V600E, panels A and B; NRAS Q61R, panel C). Grey dots correspond to empty wells.
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .
Techniques: Control, Mutagenesis, Sequencing